How To Add Gfp Into My Plasmid

pGEM-GFP-URA3-GFP
(Plasmid #72606)

• Purpose

  Template for creating a C-last GFP tag AND an internal GFP tag from a single transfection of yeast

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	72606	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add together to Cart

This material is available to academics and nonprofits just.

Backbone

• Vector backbone

  pGEM-5Zf(+) [cut with EcoR Five and T-tailed to give pGEM-T]

• Backbone manufacturer

  Promega
• Backbone size west/o insert (bp) 2964
• Total vector size (bp) 5300

• Modifications to courage

  Office of the pGEM multiple cloning site (between the EcoR 5 and Apa I sites) was replaced with inserts

• Selectable markers

  URA3

Growth in Bacteria

• Bacterial Resistance(s)

  Ampicillin

• Growth Temperature

  37°C

• Growth Strain(southward)

  DH5alpha

• Growth instructions

  Submitted plasmid was grown in Lucigen E. coli strain "Due east. cloni 10G"

• Copy number

  High Re-create

Gene/Insert 1

• Factor/Insert name

  GFP (full)

• Alt name

  Yeast enhanced green fluorescent protein three

• Species

  Aequorea victoria

• Insert Size (bp)

  717

• Mutation

  S65G, S72A

• GenBank ID

  Accession Number U73901

Factor/Insert 2

• Gene/Insert proper noun

  URA3

• Alt name

  OMP decarboxylase

• Species

  Candida albicans strain 3153A

• Insert Size (bp)

  1383

• Mutation

  Includes full 819 bp coding sequence of URA3, 435 upstream bases, and 129 downstream bases

Gene/Insert 3

• Cistron/Insert name

  GFP (fractional)

• Species

  Aequorea victoria

• Insert Size (bp)

  199

• Mutation

  Includes nucleotides 501-699 of the 717 nucleotide coding sequence of GFP

Factor/Insert 4

• Gene/Insert name

  Optional Linker

• Species

  Candida albicans

• Insert Size (bp)

  24

• Mutation

  This is a segment of the YWP1 gene that encodes SSASPSGS.

Resource Information

• Supplemental Documents

  • Addgene 72606 pGEM-GFP-URA3-GFP updated Jan 2018.pdf

• A portion of this plasmid was derived from a plasmid made past

  The yeast enhanced green fluorescent protein 3 ("GFP") portion of this plasmid was obtained from Brendan Cormack (Cormack, Bertram, Egerton, Gow, Falkow & Brown, Microbiology 143: 303-311, 1997); information technology has the S65G and S72A mutations, is codon-optimized for Candida albicans, and is similar to GenBank Accession Number U73901.

• Article Citing this Plasmid

  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms

  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials

• Industry Terms

  • Non Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

• For your Materials & Methods section:

  pGEM-GFP-URA3-GFP was a gift from Bruce Granger (Addgene plasmid # 72606 ; http://n2t.net/addgene:72606 ; RRID:Addgene_72606)

• For your References department:

  Accessibility and contribution to glucan masking of natural and genetically tagged versions of yeast wall poly peptide 1 of Candida albicans. Granger BL. PLoS One. 2018 January 12;thirteen(1):e0191194. doi: 10.1371/periodical.pone.0191194. eCollection 2018. PONE-D-17-36468 [pii] PubMed 29329339

How To Add Gfp Into My Plasmid,

Source: https://www.addgene.org/72606/

Posted by: abelalowely1959.blogspot.com

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